baumannii, four (66.7%) belonged to CC25 ( bla OXA−64), one (16.7%) to CC2 ( bla OXA−66) and one to CC3 ( bla OXA−71). One isolate belonged to CC10 ( bla OXA−68), one to CC149 ( bla OXA−104), the remaining isolate was assigned to ST1220 and possessed bla OXA−116. Six (12%) isolates belonging to CC2 and carrying bla OXA−66 were also noted. Isolates belonging to CC25 possessed bla OXA−64. Strains of IC I harbored bla OXA−69, aac(3′ )-la, aadA1, sul1, intI1, and splA/T genes. baumannii isolates from animals, two predominant clones were observed linked to CC1 ( n = 27/54% of the isolates) and CC25 ( n = 14/28%), respectively. Susceptibility profiles were determined by disc diffusion or by broth microdilution. Genes encoding bla OXA−51-like carbapenemases were amplified by PCR and sequenced. Genes encoding carbapenemases, aminoglycoside-modifying enzymes, macrolide-, quinolone- and co-trimoxazole resistance genes, the IS Aba1 element, virulence associated intI1 genes and plasmid associated toxin-antitoxin markers were identified by microarray. Assignment to sequence types (ST) and international complexes (IC) was done by multilocus sequence typing (MLST) according to the Pasteur scheme. Methods: Fifty-eight ACB-complex strains from animals treated at a veterinary clinic between 20, and seven strains collected from the hospital environment during 2012 were analyzed. TAG Theoretical and Applied Genetics Springer Journals Objectives: We investigated a collection of strains belonging to the Acinetobacter calcoaceticus- Acinetobacter baumannii (ACB) complex obtained from a veterinary clinic with regard to their genetic relatedness, presence of antibiotic resistance genes and antimicrobial susceptibility profiles. Implications for risk assessment of genetically modified wheat are discussed. Subsequently these markers were used to characterise introgression of wheat DNA into a BC1S1 family. Successfully located RAPD fragments were then converted into highly specific and easy-to-use sequence characterised amplified regions (SCARs) through sequencing and primer design. Their presence indicates that DNA from any of the wheat genomes can introgress into Ae. Introgressed fragments were not limited to the D genome of wheat, but specific fragments of A and B genomes were also present in the BC1. cylindrica chromosome number or one supernumerary chromosome, were assigned to wheat chromosomes using Chinese Spring nulli-tetrasomic wheat lines. cylindrica as the recurrent parent and having a euploid Ae. Wheat random amplified polymorphic DNA (RAPD) fragments, detected in intergeneric hybrids and introgressed to the first backcross generation with Ae. cylindrica because during meiosis in pentaploid hybrids, A and B genome chromosomes form univalents and tend to be eliminated whereas recombination takes place only in D genome chromosomes. It has been proposed that the A and B genome of bread wheat are secure places to insert transgenes to avoid their introgression into Ae. cylindrica share the D genome, issued from the common diploid ancestor Aegilops tauschii Coss. Introgression from allohexaploid wheat ( Triticum aestivum L., AABBDD) to allotetraploid jointed goatgrass ( Aegilops cylindrica Host, CCDD) can take place in areas where the two species grow in sympatry and hybridize. hybrids Introgression of wheat DNA markers from A, B and D genomes in early generation progeny of. Introgression of wheat DNA markers from A, B and D genomes in early generation progeny of Aegilops cylindrica Host × Triticum aestivum L.
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